ABSTRACT
Objective: This study was designed to evaluate whether chronic Rosa canina [RC] extract administration could improve recognition memory and depressive-like behavior in diabetic mice
Materials and Methods: Seventy-five male albino mice [25-30 g] were randomly divided into 5 groups [15 in each group]
A single intraperitoneal injection of 200 nng/ kg streptozotocin [STZ] was administered to the mice to induce diabetes
The control group received normal saline, and the diabetic groups received normal saline or 50, 250, and 500 mg/kg of RC extract for 28 days
The mice were weighed each week
Recognition memory and depressive-like behavior were assessed using forced swimming and novel object recognition [NOR] tests, respectively. Malondi-aldehyde [MDA] levels and total antioxidant capacity [TAG]were measured in the mouse brain homogenate to evaluate oxidative stress. Statistical analysis was conducted using SPSS, version 22
Results: The groups receiving 250 or 500 mg/kg RC had significantly lower immobility time [159.4 +/- 4.7 and 150.1 +/- 3.1 s] compared to the sham control group [192.1 +/- 7.8 s] in the forced swimming test, and a higher discrimination index [0.39 +/- 0.02 and 0.48 +/- 0.03] was seen in diabetic animals in the NOR task compared to the sham control group [0.2 +/- 0.01]
Also, the groups receiving treatment with RC [250 and 500 mg/kg] had significantly higher TAG [0.92 +/- 0.04 and 0.96 +/- 0.05 mmol/L] and lower MDA [0.76 +/- 0.02 and 0.67 +/- 0.03 nmol/mg protein] levels in the brains in comparison to the model group. In the 3rd and 4th weeks of study, the RC-treated mice [250 and 500 mg/kg] gained more weight [31.2 +/- 0.3 and 32.4 +/- 0.3 g, and 31.3 +/- 0.2 and 33.7 +/- 0.3 g, respectively] than the diabetic group [30 +/- 0.2 and 29.6 +/- 0.3 g]
Conc/us/on:This study showed that RG attenuated impairment of recognition memory and depressive-like behavior probably through modulation of oxidative stress in an STZ model of diabetes in mouse brains